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Your Position: 홈 > Protein > Glypican 3 > GP3-H5223

Human Glypican 3 / GPC3 (S359F) Protein, His Tag, low endotoxin

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS ,SGB,SGBS,SGBS1
  • Source
    Human Glypican 3 (S359F) Protein, His Tag(GP3-H5223) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1 (S359F)).
    Predicted N-terminus: Gln 25
    Request for sequence
  • Molecular Characterization
    Glypican 3 Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 61.7 kDa. The protein migrates as 66-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Glypican 3 SDS-PAGE

Human Glypican 3 (S359F) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Glypican 3 ELISA

Immobilized Human Glypican 3 (S359F) Protein, His Tag (Cat. No. GP3-H5223) at 0.5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human GPC3 Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

Protocol
Glypican 3 ELISA

Immobilized Human Glypican 3 (S359F) Protein, His Tag (Cat. No. GP3-H5223) at 0.5 μg/mL (100 μL/well) can bind Anti-Human GPC3 Mab with a linear range of 0.4-3 ng/mL (Routinely tested).

Protocol
Bioactivity-SPR
Glypican 3 SPR

Anti-Human GPC3 MAb (chimeric mouse-human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human Glypican 3 (S359F) Protein, His Tag (Cat. No. GP3-H5223) with an affinity constant of 1.96 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol
Bioactivity-BLI
Glypican 3 BLI

Loaded Anti-Human GPC3 MAb (chimeric mouse-human IgG1) on AHC Biosensor, can bind Human Glypican 3 (S359F) Protein, His Tag (Cat. No. GP3-H5223) with an affinity constant of 3.04 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol
  • Background
    Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.
  • Clinical and Translational Updates

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