5e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of APC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-AY54A1) and isotype control antibody respectively. APC signal was used to evaluate the binding activity (QC tested).
5e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Cat. No. FM3-PY54A2) and isotype control antibody respectively. PE signal was used to evaluate the binding activity (QC tested).
Human CD19 (20-291), Fc Tag (Cat. No. CD9-H5251) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) with an affinity constant of 0.668 nM as determined in a SPR assay (Biacore 8K) (QC tested).
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