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Recombinant Antibody Services

Recombinant Antibody Services
Background

Antibodies are widely used in biomedical research, not only as therapeutic drugs, but also in immunodiagnostics and various biologics-based analytical research. Recombinant antibodies, unlike the traditional hybridoma antibodies, have become popularized due to its unique advantages in customization and large-scale antibody production. Through genetic engineering, antibodies are developed directly from an antibody gene sequence to eliminate traditional immunization and antibody screening steps, resulting in high-throughput, large-scale antibody production.

As a supplier of key reagents in the global biomedical field, ACROBiosystems develops and produces high-quality recombinant antibody reagents to meet the growing demand for custom antibody reagents in early drug discovery and preclinical research. Relying upon ACROBiosystem’s independently-optimized mammalian cell expression platform and production experience in antibody reagent products, we provide our customers with recombinant antibody expression and production services based on HEK293 and CHO cells. We provide a comprehensive recombinant antibody development service including  gene construction, expression, purification, and quality inspection. This means starting from the sequence information of antibodies (or VH/VL) and delivering your custom recombinant antibody with high purity and low endotoxin level.

Service Scope

High-throughput Recombinant Antibody Expression Service


• CHO/HEK293-based  Cell Expression Platform
• 2-3 weeks to delivery of purified antibodies.
• Application-based customizable service including production method, concentration, etc.

Antibody Class Switching Service


• One-stop service from gene construction to expression from antibody (or VH/VL) sequence information.
• Antibody class switching services are available. (e.g. switching from hybridoma mouse antibody to human antibody IgG1/2/3/4).

Large-scale Recombinant Antibody Production Service


• Large-scale Production services supporting gram to kilogram of recombinant antibodies
• Low Endotoxin levels:<1EU/mg.
• 50L-200L Bioreactors
• Customizable production plan to support the customer needs.

Development process

Sequence information

Gene synthesis and Vector construction

Transfection and Screening

Expression evaluation

Culture

Purification

Antibody delivery

Gene synthesis
(optional)
~1 week

• Codon optimization

• Gene synthesis

Vector construction
~1 week
 

• Vector construction

• Plasmid extraction

Antibody expression
and purification
1 week

• Cell transient transfection

• Antibody expression and purification

Quality control
and delivery
3 week

• Purified recombinant antibody

• Quality inspection report, CoA

Gene synthesis
(optional)
~1 week

Codon optimization

Gene synthesis

Vector construction
~1 week
 

Vector construction

Plasmid extraction

Antibody expression
and purification
1 week

Cell transient transfection

Antibody expression and purification

Quality control
and delivery
3 week

Purified recombinant antibody

Quality inspection report, CoA

Why ACROBiosystems?

Optimized mammalian cell expression platform:
HEK293/CHO cells

Multiplexed Antibody Development:
>1000 antibody projects can be performed in parallel

High quality standard Purity:
>95% (SDS-PAGE)

High-throughput:
2-3 weeks to delivery

Flexible Deliverables:
1mg to 1g of purified recombinant antibody.

Large-scale Production Capacity:
From gram to kilogram to meet industrial demand

Powerful Analytical and Detection Technology Platforms:
SEC-MALS/UNcle/Biacore/Fortebio

Case Studies
Recombinant Antibody Expression using HEK293 cells
Isotype
Human IgG1/kappa
Purity
>95% as determined by SDS-PAGE.
Isotype
Human IgG3/kappa
Purity
>95% as determined by SDS-PAGE.
Isotype
Mouse IgG1/kappa
Purity
>95% as determined by SDS-PAGE.
Bioactivity

Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Human IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M2) with a linear range of 1-10 ng/mL (QC tested).

Bioactivity

Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Human IgG3 Kappa Isotype Control (mAb) (Cat. No. DNP-M915) with a linear range of 0.1-4 ng/mL (QC tested).

Bioactivity

Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Mouse IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M1) with a linear range of 2-20 ng/mL (QC tested).

Recombinant Antibody Expression using CHO cells
Expression result
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
SourceIsotypePurityEndotoxin
CHO cellsHuman IgG1/kappa>95% as determined
by SDS-PAGE
<0.1EU/mg

Inquiry and order

  • ACROBiosystems scientist will respond within 24 hours of submission.

  • Call us at: +1 800-810-0816 or email at order@acrobiosystems.com for consultation.

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