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> Cytokine detection ELISA kits
Quick & Easy-to-use: 2 hour assay with only 7 steps required.
Consistent: Calibrated using NIBSC/WHO standards to ensure batch-to-batch consistency.
Strict Quality Control: High sensitivity, specificity, accuracy, linearity and inter/intra precision.
Validated Method: Comprehensive validation with available protocol and 24h professional technical support.
Minimal Matrix Interference: Low matrix interference verified in cell culture supernatant and serum.
| Cat. No. | Product Description |
|---|
| RUO | PRO | ||
|---|---|---|---|
| Detection Time | 2-3h | 2h | |
| Sample Type | Cell Culture Supernates, Serum , Plasma | Cell Culture Supernates, Serum , Plasma | |
| Quality Management System | ISO13485 | ||
| Validation Guidance | ICH M10 | ||
| Validation Experiment | Hook effect | √ | √ |
| Matrix effect | Bilirubin, Hemoglobin, Triglyceride | ||
| Anticoagulant effect | EDTA, Heparin, Sodium citrate | ||
| Influence of sample storage conditions | 3 Freeze–thaw Cycles | ||
| Specificity | IL-2, IL-4, IL-6, IL-7, IL-10, IL-15, IL-21, IL-1β, TNF-alpha, GM-CSF | IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 p70, IL-13, IL-15, IL-17, GM-CSF, TNF-alpha, IFN-gamma, etc. | |
The sensitivity of ClinMax™ Human Interferon-γ (IFN-γ) ELISA Kit (Cat. No. CRS-A017) is 5.309 pg/Ml.
ClinMax™ Human Interferon-γ (IFN-γ) ELISA Kit (Cat. No. CRS-A017) was verified to have an intra/inter-precision of less than 10%.
The sensitivity of ClinMax™ Human IFN-γ ELISA Kit, PRO (Cat. No. CEA-C006) is 3.25 pg/mL.
ClinMax™ Human IFN-γ ELISA Kit, PRO (Cat. No. CEA-C006) was verified to have an intra/inter-precision of less than 10%.
Diseases that are commonly present during sample collection such as jaundice (elevated bilirubin), hemolysis (elevated hemoglobin), and lipemia (elevated triglyceride) were simulated. Bilirubin, hemoglobin, and triglycerides were individually spiked into the sample matrix at a concentration of 20 mg/dL, 3500 g/dL, 2.0 g/L, respectively to evaluate matrix effect on our (XXX) kits.
Anticoagulant effect was tested by spiking the sample with either heparin, EDTA, or sodium citrate at a concentration of 40U/mL, 4mg/mL, and 40mg/mL, respectively.
| IFN-γ Standard | OD1 | OD2 | AVERAGE | ODAVERAGE-BLANK |
|---|---|---|---|---|
| 0pg/mL(Blank) | 0.0583 | 0.0472 | 0.05275 | 0 |
| 19.5pg/mL | 0.084 | 0.081 | 0.0825 | 0.02975 |
| 39.1pg/mL | 0.1324 | 0.1403 | 0.13635 | 0.0836 |
| 78.1pg/mL | 0.2442 | 0.2253 | 0.23475 | 0.182 |
| 156.25pg/mL | 0.427 | 0.4125 | 0.41975 | 0.367 |
| 312.5pg/mL | 0.7573 | 0.7332 | 0.74525 | 0.6925 |
| 625pg/mL | 1.3271 | 1.3245 | 1.3258 | 1.27305 |
| 1250pg/mL | 2.2102 | 2.1864 | 2.1983 | 2.14555 |
| Samples | OD1 | OD2 | AVERAGE | ODAVERAGE-BLANK | IFN-γ content（pg/mL） |
|---|---|---|---|---|---|
| 1 | 0.752 | 0.7561 | 0.75405 | 0.7013 | 313.853045 |
| 2 | 0.105 | 0.1045 | 0.10475 | 0.052 | 27.128759 |
| 3 | 0.092 | 0.0971 | 0.09455 | 0.0418 | 23.219143 |
| 4 | 0.0952 | 0.1003 | 0.09775 | 0.045 | 24.442757 |
| 5 | 0.1008 | 0.1026 | 0.1017 | 0.04895 | 25.956882 |
| 6 | 0.112 | 0.0994 | 0.1057 | 0.05295 | 27.494249 |
| 7 | 0.0994 | 0.0959 | 0.09765 | 0.0449 | 24.404478 |
| 8 | 0.3364 | 0.3455 | 0.34095 | 0.2882 | 123.398059 |
| 9 | 0.1995 | 0.1846 | 0.19205 | 0.1393 | 61.534605 |
| 10 | 0.136 | 0.1409 | 0.13845 | 0.0857 | 40.223079 |
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