제품 수집
제품 세부정보
Synonyms
DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNFα
Source
Rat TNF-alpha Protein, His Tag, active trimer (TNA-R52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 80 - Leu 235 (Accession # P16599).
Predicted N-terminus: Leu 80
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.1 kDa. The protein migrates as 18-19 kDa and 24-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a trimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
고객님도 확인한 상품
데이터 표시
SDS-PAGE
Rat TNF-alpha Protein, His Tag, active trimer on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Rat TNF-alpha Protein, His Tag, active trimer (Cat. No. TNA-R52H3) is more than 90% and the molecular weight of this protein is around 58-73 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human TNFR1, Fc Tag (Cat. No. TN1-H5251) at 5 μg/mL (100 μL/well) can bind Rat TNF-alpha Protein, His Tag, active trimer (Cat. No. TNA-R52H3) with a linear range of 0.5-31 ng/mL (QC tested).
Protocol
Immobilized Human TNFR2 / CD120b / TNFRSF1B Protein, Fc Tag (Cat. No. TN2-H5253) at 5 μg/mL (100 μL/well) can bind Rat TNF-alpha Protein, His Tag, active trimer (Cat. No. TNA-R52H3) with a linear range of 1-63 ng/mL (Routinely tested).
Protocol
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배경 소개
프론티어 진행
의약품 개발 진행- 영어 이름：
Tumor necrosis factor α
- 카테고리：
- 상장 의약품 수：
68 상세
- 임상 의약품 수：
74 상세
- 최고 개발 단계：
Approved
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전화
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