Streptavidin (A24C) Protein, His Tag

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Cat. No. / Size
Price
Qty
STN-N51H3-1mg
$180.00
STN-N51H3-5mg (1mg X 5)
$670.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Streptavidin, SA

  • Source

    Streptavidin (A24C) Protein, His Tag (STN-N51H3) is expressed from E. coli cells. It contains AA Cys 24 - Gln 183 (Accession # P22629-1 (A24C)).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    Streptavidin Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 18.6 kDa. The protein migrates as 20-22 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Streptavidin SDS-PAGE

    Streptavidin (A24C) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     Streptavidin ELISA

    Immobilized Biotinylated Human Dkk-1, Fc,Avitag (Cat. No. DK1-H82F5) at 5 μg/mL (100 μL/well) on Streptavidin (A24C) Protein, His Tag (Cat. No. STN-N51H3) precoated (0.5 μg/well) plate can bind Human LRP-5, Mouse IgG2a Fc Tag (Cat. No. LR5-H5254) with a linear range of 0.5-16 ng/mL (QC tested).

    Protocol

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Background

Streptavidin is a 66KDa tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity to biotin. Each unit can bind one biotin. Horseradish peroxidase is metalloenzyme, a 44KDa glycoprotein. When incubate with substrates, it produces a coloured, fluorimetric, or luminescent derivatives, which can be detected and quantified. HRP conjugated Streptavidin is widely used for the detection and quantification of biotinylated proteins.

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