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Product Details
Synonyms
CD33, SIGLEC3, gp67
Source
Alexa Fluor 647-Labeled Human Siglec-3, His Tag (CD3-HA2H6) is produced via conjugation of AF647 to Human Siglec-3, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human Siglec-3, His Tag is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1).
Predicted N-terminus: Asp 18
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
Conjugate
AF647
Excitation Wavelength: 640 nm
Emission Wavelength: 672 nm
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
Evaluation of CAR expression
FACS Analysis of Anti-Siglec-3 CAR Expression
5e5 of 293 CAR cells transfected with anti-Siglec-3-scFv were stained with 100 μL of 3 μg/mL of Alexa Fluor 647-Labeled Human Siglec-3, His Tag (Cat. No. CD3-HA2H6) and negative control protein respectively (Fig. C and B), and non- transfected 293 cells were used as a control (Fig. A), Alexa 647 signal was used to evaluate the binding activity (QC tested).
ProtocolFACS Analysis of Non-specific binding to PBMCs
5e5 of PBMCs were stained with Alexa Fluor 647-Labeled Human Siglec-3, His Tag (Cat. No. CD3-HA2H6) and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in PBMCs, and Alexa 647 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Myeloid cell surface antigen CD33
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
44 Details
- Highest Development Stage：
Approved
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