Save Product
Product Details
Synonyms
IL-23 alpha & IL-12 beta
Source
Human IL-23 alpha&Mouse IL-12 beta Heterodimer Protein, His Tag&Tag Free (ILB-HM52W6) is expressed from human 293 cells (HEK293). It contains AA Arg 20 - Pro 189 (IL23A) & Met 23 - Ser 335 (IL12B) (Accession # Q9NPF7-1 (IL23A) & P43432-1 (IL12B)).
Predicted N-terminus: His (IL23A) & Met 23 (IL12B)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Human IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL-23 alpha and IL-12 beta, has a calculated MW of 19.5 kDa (IL-23 alpha) and 35.8 kDa (IL-12 beta). Subunit IL-23 alpha is fused with a polyhistidine tag at the N-terminus and subunit IL-12 beta contains no tag. The protein migrates as 20 kDa (IL23A) & 45 kDa (IL12B) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Human IL-23 alpha&Mouse IL-12 beta Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IL-23 alpha&Mouse IL-12 beta Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HM52W6) is more than 85% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human IL-23 alpha&Mouse IL-12 beta Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HM52W6) at 2 μg/mL (100 μL/well) can bind Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) with a linear range of 5-78 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Human IL-23 R, Fc Tag (Cat. No. ILR-H5254) on Protein A Biosensor, can bind Human IL-23 alpha&Mouse IL-12 beta Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HM52W6) with an affinity constant of 15.7 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
Loaded Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) on Protein A Biosensor, can bind Human IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HM52W6) with an affinity constant of 2.79 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Bioactivity-CELL BASE
Human IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HM52W6) stimulates secretion of IL-17 by Mouse spleen cells. The ED50 for this effect is 0.0069-0.0426 ng/mL (Routinely tested).
Protocol
Cell based assay shows that the secretion of IL-17 induced by Human IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HM52W6) is inhibited by increasing concentration of the Anti-Human IL-23 neutralizing antibody. The IC50 is between 0.41-1.012 ng/mL (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Drug Development Progress- English Name：
Interleukin-12 subunit beta
- Category：
- Approved Drugs：
7 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Approved
Contact Us
전화
글로벌: +1 800-810-0816 (무료)
아시아태평양: +86 400-682-2521
Drug Candidate Licensing
