제품 수집
제품 세부정보
Synonyms
IL-23 alpha & IL-12 beta
Source
Biotinylated Cynomolgus IL-23 alpha&IL-12 beta Heterodimer Protein, His,Avitag&Tag Free (ILB-C82W3) is expressed from human 293 cells (HEK293). It contains AA Val 22 - Pro 189 & Ile 23 - Ser 328 (Accession # G7PIH8 & G7P6S2).
Predicted N-terminus: His & Ile 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Biotinylated Cynomolgus IL-23 alpha&IL-12 beta Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of IL23A and IL12B, has a calculated MW of 22 kDa (IL23A) & 34.8 kDa (IL12B). Subunit IL23A is fused with an Avi tag (Avitag™), followed by a polyhistidine tag at the N-terminus and subunit IL12B contains no tag. The protein migrates as 25-27 kDa, 35-38 kDa and 40-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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데이터 표시
SDS-PAGE
Biotinylated Cynomolgus IL-23 alpha&IL-12 beta Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Cynomolgus IL-23 alpha&IL-12 beta Heterodimer Protein, His,Avitag&Tag Free (Cat. No. ILB-C82W3) is more than 90% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Cynomolgus IL-23 alpha&IL-12 beta Heterodimer Protein, His,Avitag&Tag Free (Cat. No. ILB-C82W3) at 0.5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Cynomolgus IL-23 R, Fc Tag (Cat. No. ILR-C5251) with a linear range of 0.05-3 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Cynomolgus IL-23 alpha&IL-12 beta Heterodimer Protein, His,Avitag&Tag Free (Cat. No. ILB-C82W3) at 0.5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Guselkumab (CNTO 1959) with a linear range of 0.05-2 ng/mL (Routinely tested).
Protocol
Immobilized Biotinylated Cynomolgus IL-23 alpha&IL-12 beta Heterodimer Protein, His,Avitag&Tag Free (Cat. No. ILB-C82W3) at 0.5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human IL23A&IL12B P40 domain Antibody, Human IgG1 with a linear range of 0.02-1 ng/mL (Routinely tested).
Protocol
Immobilized Human IL-23 R, Fc Tag (Cat. No. ILR-H5254) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus IL-23 alpha&IL-12 beta Heterodimer Protein, His,Avitag&Tag Free (Cat. No. ILB-C82W3) with a linear range of 2-63 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human IL-23 R, Fc Tag (Cat. No. ILR-H5254) captured on Protein A Chip can bind Biotinylated Cynomolgus IL-23 alpha&IL-12 beta Heterodimer Protein, His,Avitag&Tag Free (Cat. No. ILB-C82W3) with an affinity constant of 1.21 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Cynomolgus IL-23 R, Fc Tag (Cat. No. ILR-C5251) captured on Protein A Chip can bind Biotinylated Cynomolgus IL-23 alpha&IL-12 beta Heterodimer Protein, His,Avitag&Tag Free (Cat. No. ILB-C82W3) with an affinity constant of 15.1 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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배경 소개
프론티어 진행
의약품 개발 진행- 영어 이름：
Interleukin-12 subunit beta
- 카테고리：
- 상장 의약품 수：
7 상세
- 임상 의약품 수：
2 상세
- 최고 개발 단계：
Approved
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전화
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