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Product Details
Synonyms
IL-4 R alpha & IL-13 R alpha 1, IL4RA & IL13RA1
Source
Rat IL-4 R alpha & IL-13 R alpha 1 Protein, His Tag&His Tag (ILR-R52H5) is expressed from human 293 cells (HEK293). It contains AA Ile 26 - Arg 232 & Thr 27 - Thr 342 (Accession # Q63257-1 & Q561K3).
Predicted N-terminus: Ile 26 & Thr 27
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Rat IL-4 R alpha & IL-13 R alpha 1 Protein, His Tag&His Tag, produced by co-expression of IL-4 R alpha and IL-13 R alpha 1, has a calculated MW of 29.1 kDa (IL-4 R alpha) and 41 kDa (IL-13 R alpha 1). Subunit IL-4 R alpha is fused with a polyhistidine tag at the C-terminus, and subunit IL-13 R alpha 1 contains a polyhistidine tag at the C-terminus. The protein migrates as 43-53 kDa and 60-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Rat IL-4 R alpha & IL-13 R alpha 1 Protein, His Tag&His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Rat IL-4 R alpha & IL-13 R alpha 1 Protein, His Tag&His Tag (Cat. No. ILR-R52H5) is more than 90% and the molecular weight of this protein is around 90-110 kDa verified by SEC-MALS.
Report
Bioactivity-SPR
Mouse IL-4, His Tag (Cat. No. IL4-M52H5) immobilized on CM5 Chip can bind Rat IL-4 R alpha & IL-13 R alpha 1 Protein, His Tag&His Tag (Cat. No. ILR-R52H5) with an affinity constant of 8.80 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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