Human IL-2 Protein, His Tag

  • Related Products: TR-FRET kit FRT-C03
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제품 번호/규격
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IL2-H52H8-100ug
$325.00
IL2-H52H8-1mg (500ug X 2)
$2360.00
ETA of in-stock products:2 business days
총 항목 수 제품 금액$ 0

제품 세부정보

  • Synonyms

    IL2, TCGF, lymphokine, Interleukin 2

  • Source

    Human IL-2, His Tag (IL2-H52H8) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 153 (Accession # P60568-1).

    Predicted N-terminus: Ala 21

    Request for sequence
  • Molecular Characterization

    IL-2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 17.3 kDa. The protein migrates as 18 kDa and 19 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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데이터 표시

  • SDS-PAGE

    IL-2 SDS-PAGE

    Human IL-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     IL-2 ELISA

    Immobilized Human IL-2 R alpha, Fc Tag (Cat. No. ILA-H5251) at 5 μg/mL (100 μL/well) can bind Human IL-2, His Tag (Cat. No. IL2-H52H8) with a linear range of 0.3-2.5 ng/mL (QC tested).

    Protocol
  • Bioactivity-BLI

     IL-2 BLI

    Loaded Biotinylated Human IL-2RB&IL-2RA&IL-2RG, Fc,Avitag&Fc,Avitag (Cat. No. ILG-H82F4) on SA Biosensor, can bind Human IL-2, His Tag (Cat. No. IL2-H52H8) with an affinity constant of 2.41 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

    Protocol

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배경 소개

Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotrophic hormone that is instrumental in the body's natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit crossspecies activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self antigens", which could result in "autoimmunity". T-Regs do so by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.

프론티어 진행

 
의약품 개발 진행
  • 영어 이름:

    Interleukin-2

  • 카테고리:

  • 상장 의약품 수:

    2 상세

  • 임상 의약품 수:

    46 상세

  • 최고 개발 단계:

    Approved

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