Human Glypican 4 / GPC4 Protein, His Tag (MALS verified)

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Cat. No. / Size
Price
Qty
GP4-H52H3-100ug
$390.00
GP4-H52H3-1mg (250ug X 4)
$2680.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Glypican 4, glypican proteoglycan 4, glypican-4, GPC4, K-glypican

  • Source

    Human Glypican 4, His Tag (GP4-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Ser 529 (Accession # O75487-1).

    Request for sequence
  • Molecular Characterization

    Glypican 4 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 59.5 kDa. The protein migrates as 60-65 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Glypican 4 SDS-PAGE

    Human Glypican 4, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    Glypican 4 SEC-MALS

    The purity of Human Glypican 4, His Tag (Cat. No. GP4-H52H3) is more than 90% and the molecular weight of this protein is around 55-69 kDa verified by SEC-MALS.

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  • Bioactivity-ELISA

     Glypican 4 ELISA

    Immobilized Human FGF basic, premium grade (Cat. No. BFF-H4117) at 1 μg/mL (100 μL/well) can bind Human Glypican 4, His Tag (Cat. No. GP4-H52H3) with a linear range of 0.02-0.625 μg/mL (QC tested).

    Protocol

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Background

Glypican-4 is a protein that in humans is encoded by the GPC4 gene. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome.

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