Product Details
Synonyms
BXMAS1, CD307, CD307e, FCRH5, IRTA2, PRO820
Source
Biotinylated Human FCRL5, His,Avitag (FC5-H82E3) is expressed from human 293 cells (HEK293). It contains AA Gln 16 - Gly 851 (Accession # Q96RD9-1).
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 95.0 kDa. The protein migrates as 105-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Biotinylated Human FCRL5, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS

The purity of Biotinylated Human FCRL5, His,Avitag (Cat. No. FC5-H82E3) is more than 85% and the molecular weight of this protein is around 100-120 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Biotinylated Human FCRL5, His,Avitag (Cat. No. FC5-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-Human CD307e/FCRL5 Antibody(RG-7598) with a linear range of 0.03-1 ng/mL (QC tested).
Protocol
Bioactivity-SPR

Biotinylated Human FCRL5 Protein, His,Avitag (Cat. No. FC5-H82E3) capture on NTA-Series S sensor chip can bind Ipilimumab with an affinity constant of 18.5 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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Background
Recent Advances
- English Name:
Fc receptor-like protein 5
- Category:
- Approved Drugs:
0 Details
- Drugs in Clinical Trials:
3 Details
- Highest Development Stage:
Phase 2 Clinical
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