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Product Details
Synonyms
FAP, FAPalpha, SIMP, Seprase, APCE
Source
Mouse FAP, His Tag (FAP-M52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 761 (Accession # P97321-1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 87.1 kDa. The protein migrates as 80-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Mouse FAP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse FAP, His Tag (Cat. No. FAP-M52H3) is more than 90% and the molecular weight of this protein is around 180-190 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse FAP, His Tag (Cat. No. FAP-M52H3) at 1 μg/mL (100 μL/well) can bind Anti-FAP Antibody, Mouse IgG2a (FAP5) with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Anti-FAP Antibody, Mouse IgG2a (FAP5) captured on CM5 chip via anti-mouse antibodies surface can bind Mouse FAP, His Tag (Cat. No. FAP-M52H3) with an affinity constant of 1.94 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity
Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >6500 pmol/min/µg (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Prolyl endopeptidase FAP
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
88 Details
- Highest Development Stage：
Phase 3 Clinical
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