Biotinylated Rat CD3 epsilon&CD3 gamma Heterodimer Protein, His,Avitag™&Flag Tag (MALS verified)

Synonyms

CD3 epsilon & CD3 gamma, CD3E & CD3G

Source

Biotinylated Rat CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (CDG-R82D4) is expressed from human 293 cells (HEK293). It contains AA Gln 21 - Asp 103 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # NP_001101610.1 (CD3E) & NP_001071114.1 (CD3G)).

Predicted N-terminus: Gln 21 (CD3E) & Gln 23 (CD3G)

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

Biotinylated Rat CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 16.7 kDa (CD3E) and 15.7 kDa (CD3G). Subunit CD3E carries a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G is fused with a flag tag at the C-terminus. As a result of glycosylation, the protein migrates as 22-25 kDa under reducing (R) condition, and 40-45 kDa under non-reducing (NR) condition (SDS-PAGE).

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the HABA assay / binding ELISA.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process