Human CD3 gamma / CD3G Protein, Fc Tag (MALS verified)

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Cat. No. / Size
Price
Qty
CDA-H5254-100ug
$360.00
CDA-H5254-1mg
$2615.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    CD3G, CD3-GAMMA, IMD17, T3G, TCR gamma

  • Source

    Human CD3 gamma, Fc Tag (CDA-H5254) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Ser 116 (Accession # P09693-1).

    Predicted N-terminus: Gln 23

    Request for sequence
  • Molecular Characterization

    CD3 gamma Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 37.1 kDa. The protein migrates as 45-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    CD3 gamma SDS-PAGE

    Human CD3 gamma, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    CD3 gamma SEC-MALS

    The purity of Human CD3 gamma, Fc Tag (Cat. No. CDA-H5254) is more than 85% and the molecular weight of this protein is around 80-90 kDa verified by SEC-MALS.

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Background

CD3 gamma chain is part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface. Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G.

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