Save Product
Product Details
Synonyms
FLJ18683, T3E, TCRE, CD3E, CD3-epsilon
Source
MABSol® Biotinylated Human CD3E, His Tag, primary amine labeling (CDE-H8224) is expressed from human HEK293 cells. It contains AA Asp 23 - Asp 126 (Accession # NP_000724.1).
It is the biotinylated form of Human CD3 epsilon, His Tag (Cat. No. CDE-H5223).Predicted N-terminus: Asp 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.9 kDa. The protein migrates as 18-20 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Biotinylated Human CD3E, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human CD3E, His Tag, primary amine labeling (Cat. No. CDE-H8224) is more than 90% and the molecular weight of this protein is around 43-55 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse Anti-Human CD3 (SP34-2) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD3E, His Tag, primary amine labeling (Cat. No. CDE-H8224) with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Bispecific T-cell Engager captured on Protein A Chip can bind Biotinylated Human CD3E, His Tag, primary amine labeling (Cat. No. CDE-H8224) with an affinity constant of 0.815 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Drug Development Progress- English Name：
T-cell surface glycoprotein CD3 epsilon chain
- Category：
- Approved Drugs：
4 Details
- Drugs in Clinical Trials：
7 Details
- Highest Development Stage：
Approved
Contact Us
전화
글로벌: +1 800-810-0816 (무료)
아시아태평양: +86 400-682-2521
Drug Candidate Licensing
