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Product Details
Synonyms
CD70, CD27LG, TNFSF7, TNFSF7G, CD27L
Source
Mouse CD27 Ligand Protein, His Tag (CDL-M5245) is expressed from human 293 cells (HEK293). It contains AA Gln 47 - Pro 195 (Accession # O55237-1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 18.6 kDa. The protein migrates as 27-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Mouse CD27 Ligand Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Mouse CD27 Ligand Protein, His Tag (Cat. No. CDL-M5245) is more than 95% and the molecular weight of this protein is around 70-90 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse CD27 Protein, Mouse IgG2a Fc Tag (Cat. No. CD7-M5252) at 2 μg/mL (100 μL/well) can bind Mouse CD27 Ligand Protein, His Tag (Cat. No. CDL-M5245) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
CD70 antigen
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
37 Details
- Highest Development Stage：
Phase 2 Clinical
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