Product Details
Source
Monoclonal Anti-Human Phospho-Tau (Thr181) Antibody, Mouse IgG2a (8D8C10) is a mouse monoclonal antibody derived from hybridoma cells fused with SP2/0 myeloma cells and murine B lymphocytes.
Antibody Type
Hybridoma Monoclonal
Clone
8D8C10
Isotype
Mouse IgG2a, Kappa
Host Species
Mouse
Immunogen
Recombinant polypeptide protein
Specificity
Specifically recognizes Human p-tau181 Protein.
Application
ApplicationRecommended UsageIF1:50-1:500Purification
Protein A purified / Protein G purified.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Performance Data
Immunofluorescence

2D cell staining: Immunofluorescent staining (10X) of phosphorylated tau in treated SH-SY5Y neuroblastoma cells with purified PT1-Y2073 at 1:200 dilution. DAPI (blue) was used as nuclear counterstain.
Protocol
Western Blot

Detection of Monoclonal Anti-Human p-tau181 antibody,Mouse,Mouse IgG2a(8D8C10), Mouse IgG2a | Kappa,HEK by Western Blot. Monoclonal Anti-Human p-tau181 antibody,Mouse,Mouse IgG2a(8D8C10), Mouse IgG2a | Kappa,HEK at 10.0 ug/ml dilution + Phosphorylated SH-SY5Y cell lysate, Mouse Tag at 20uL.
Secondary: (MFC)-Peroxidase AffiniPure GoatAnti-MouseIgG,FcγFragmentSpecific(1:1500).
Predicted band size: 65 kDa 12% Bis-Tris Protein Gel.
The recommended dilution ratio is 10.0 ug/ml.
Customer Reviews Writing Reviews

Background
Recent Advances
- English Name:
Microtubule-associated protein tau
- Category:
- Approved Drugs:
1 Details
- Drugs in Clinical Trials:
46 Details
- Highest Development Stage:
Approved
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