Human TSLP (R127A, R130A) Protein, Mouse IgG2a Fc Tag, low endotoxin (MALS verified)

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TSP-H5255-100ug
$380.00
TSP-H5255-1mg
$2615.00
ETA of in-stock products:2 business days
총 항목 수 제품 금액$ 0

제품 세부정보

  • Synonyms

    TSLP

  • Source

    Human TSLP (R127A, R130A) Protein, Mouse IgG2a Fc Tag (TSP-H5255) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # Q969D9-1 (R127A, R130A)).

    Predicted N-terminus: Tyr 29

    Request for sequence
  • Molecular Characterization

    TSLP Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 41.6 kDa. The protein migrates as 50-58 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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데이터 표시

  • SDS-PAGE

    TSLP SDS-PAGE

    Human TSLP (R127A, R130A) Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    TSLP SEC-MALS

    The purity of Human TSLP (R127A, R130A) Protein, Mouse IgG2a Fc Tag (Cat. No. TSP-H5255) is more than 95% and the molecular weight of this protein is around 85-110 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     TSLP ELISA

    Immobilized Human TSLP R, Fc Tag (Cat. No. TSR-H525a) at 5 μg/mL (100 μL/well) can bind Human TSLP (R127A, R130A), Mouse IgG2a Fc Tag (Cat. No. TSP-H5255) with a linear range of 0.3-1 ng/mL (QC tested).

    Protocol
  • Bioactivity-FACS

     TSLP FACS

    2e5 of Human TSLP R (Luc) HEK293 Reporter Cells were stained with 100 μL of 1 μg/mL of Human TSLP (R127A, R130A) Protein, Mouse IgG2a Fc Tag (Cat. No. TSP-H5255) and negative control protein respectively, washed and then followed by PE anti-mouse IgG2a Antibody and analyzed with FACS(Routinely tested).

    Protocol

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배경 소개

Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine involved in the pathology of inflammatory skin diseases, and is widely expressed by epithelial cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Human TSLP has been shown to developing nondeletional central tolerance, amplifying epithelium-induced class switching, inducing atopic diseases and maintaining intestinal noninflammatory environment. Among diverse cells responding to Human TSLP, CD11c+ dendritic cells are the most obviously characterized target cells.

프론티어 진행

 
의약품 개발 진행
  • 영어 이름:

    Thymic stromal lymphopoietin

  • 카테고리:

  • 상장 의약품 수:

    1 상세

  • 임상 의약품 수:

    29 상세

  • 최고 개발 단계:

    Approved

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  • 배경
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