PE-Labeled Human TIGIT Protein, His TagStar Staining

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TIT-HP2H3-25tests
$360.00
TIT-HP2H3-200tests
$2245.00
ETA of in-stock products:2 business days
총 항목 수 제품 금액$ 0

제품 세부정보

  • Synonyms

    TIGIT, VSIG9, VSTM3

  • Source

    PE-Labeled Human TIGIT Protein, His Tag (TIT-HP2H3) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # Q495A1-1).

    Predicted N-terminus: Met 22

  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.2% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

고객님도 확인한 상품

데이터 표시

  • Evaluation of CAR expression

    FACS Analysis of TIGIT binding to 293T overexpressing CD155 cells

     TIGIT CAR_T

    Flow cytometric analysis of 293T overexpressing CD155 cells staining with PE-Labeled Human TIGIT Protein, His Tag (Cat. No. TIT-HP2H3) at 1:50 dilution (2 μL of the stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL), compared with negative control protein(Fig. B and A). PE signal was used to evaluate the binding activity (QC tested).

    Protocol

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배경 소개

T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

프론티어 진행

 
의약품 개발 진행
  • 영어 이름:

    T cell immunoglobulin and ITIM domains

  • 카테고리:

  • 상장 의약품 수:

    0 상세

  • 임상 의약품 수:

    41 상세

  • 최고 개발 단계:

    Phase 3 Clinical

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  • 고객님도 확인한 상품
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