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Product Details
Synonyms
SHPS1, SIRPA, CD172A, BIT, MFR, MYD1, P84, PTPNS1
Source
Human SIRP alphaV2 (31-148), Llama IgG2b Fc Tag (SI2-H5257) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Ser 148 (Accession # ATD50864.1).
Predicted N-terminus: Glu 31
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a llama IgG2b Fc tag at the C-terminus.
The protein has a calculated MW of 40.6 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human SIRP alphaV2 (31-148), Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human SIRP alphaV2 (31-148), Llama IgG2b Fc Tag (Cat. No. SI2-H5257) is more than 90% and the molecular weight of this protein is around 75-95 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human SIRP alphaV2 (31-148), Llama IgG2b Fc Tag (Cat. No. SI2-H5257) at 5 μg/mL (100 μL/well) can bind Human CD47, His Tag (Cat. No. CD7-H5227) with a linear range of 0.02-0.313 μg/mL (QC tested).
Protocol
Immobilized Human CD47, His Tag (Cat. No. CD7-H5227) at 5 μg/mL (100 μL/well) can bind Human SIRP alphaV2 (31-148), Llama IgG2b Fc Tag (Cat. No. SI2-H5257) with a linear range of 0.2-5 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Signal-regulatory protein alpha
- Category：
- Approved Drugs：
2 Details
- Drugs in Clinical Trials：
19 Details
- Highest Development Stage：
Approved
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