Human Siglec-7 / CD328 Protein, Fc (L118A, G120A, E201A) Tag, low Endotoxin (MALS verified)

Synonyms

CDw328, D-siglec, A79 membrane protein, p75, Adhesion inhibitory receptor molecule 1, AIRM-1

Source

Human Siglec-7, Fc (L118A, G120A, E201A) Tag (SI7-H5257) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Leu 353 (Accession # Q9Y286-1).

Predicted N-terminus: Gln 19

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a Fc (L118A, G120A, E201A) tag at the C-terminus. The protein has a calculated MW of 63.3 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Mutations (L118A, G120A, E201A /EU number: L235A/G237A/E318A) in human immunoglobulin G1 (hIgG1) Fc strongly reduce binding of the Fc mutant to cell expressed FcγRs, resulting in an almost 4-fold reduction in ADCC compared to that of wild type human IgG1.

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process