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Product Details
Synonyms
RPL, Protein L
Source
PE-Labeled Recombinant Protein L, His Tag (RPL-PP2H2) is produced via site-specific conjugation of PE to Recombinant Protein L, His Tag under optimal conditions with a proprietary technology. Recombinant Protein L, His Tag is expressed from E. coli cells. It contains AA Lys 106 - Gly 470 (Accession # D6S9W1-1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 43.7 kDa.
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Application
Please note that this product is NOT compatible to streptavidin detection system.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
Bioactivity-FACS
5e5 of the CD19-CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Recombinant Protein L Protein, His Tag (Cat. No. RPL-PP2H2) and negative control protein respectively, PE signal was used to evaluate the binding activity (QC tested).
Protocol
5e5 of the BCMA-CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Recombinant Protein L Protein, His Tag (Cat. No. RPL-PP2H2) and negative control protein respectively, PE signal was used to evaluate the binding activity (QC tested).
Protocol
Customer Reviews Writing Reviews
1***02022-08-03
LikesThe pl antibody can detect the scfv region of most antigen CARs. We use it to detect the scfv of CD33 in our CD33 CAR-NK, and it can also be diluted with good results!
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