Recombinant Protein L, His Tag, low endotoxin

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Cat. No. / Size
Price
Qty
RPL-P3141-2mg
$125.00
RPL-P3141-10mg
$310.00
RPL-P3141-1g (10mg X 100)
$2885.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    RPL, Protein L

  • Source

    Recombinant Protein L, His Tag, also known as Recombinant Peptostreptococcus magnus Protein L, was expressed in E. coli at ACRObiosystems.

  • Molecular Characterization

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 42.2 kDa. The protein migrates as 45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 100 mM NaH2PO4, pH8.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Protein L SDS-PAGE

    Recombinant Protein L, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

Customer Reviews
Writing Reviews

  • M***D2021-09-15
    Likes
    I used recombinant protein L from ACROS Biosystems to prepare gold conjugates for immunosensors and rapid quantification of human IgG and was able to detect 2.5 ng of Human IgG in less than 1 minute. I highly recommend this product for the detection and isolation of antibodies and Fab'2 fragments. I will definitely be ordering this item again.

Background

Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

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