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Product Details
Synonyms
Prefusion glycoprotein F0/pre-F protein (RSV)
Source
Human parainfluenza viruses Pre-fusion glycoprotein F0, His Tag (PR0-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Thr 493 (Accession # AGW51052.1).
Predicted N-terminus: Gln 19
Request for sequenceMolecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 57.8 kDa. The protein migrates as 58-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Within the expressed region, several mutations were introduced to optimize the protein expression and stability.
The protein is designed as a trimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human parainfluenza viruses Pre-fusion glycoprotein F0, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human parainfluenza viruses Pre-fusion glycoprotein F0, His Tag (Cat. No. PR0-H52H3) is more than 95% and the molecular weight of this protein is around 200-230 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human parainfluenza viruses Pre-fusion glycoprotein F0, His Tag (Cat. No. PR0-H52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human parainfluenza viruses Pre-fusion glycoprotein F0 Antibody, Human IgG1 (15A10) (Cat. No. HPV-MY2325) with a linear range of 0.04-2 ng/mL (Routinely tested).
Protocol
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FAQ
- Product
What do “pre” and “post” refer to in the names of viral proteins?
In virology, “pre” refers to the conformation of a viral surface glycoprotein before fusion with the host cell membrane, while “post” refers to the conformation after membrane fusion and entry into the host cell. Both are naturally occurring states in the viral life cycle. Because the “pre” conformation represents a critical stage before viral entry, antibodies designed against this state often exhibit stronger neutralizing activity and are therefore more effective in preventing infection.
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