Product Details
Synonyms
Postfusion glycoprotein B/Postfusion gB (HSV)
Source
HSV-2 (strain 333) Glycoprotein B (gB), His Tag (GLB-H52H3) is expressed from human 293 cells (HEK293). It contains AA Glu 98 - Ala 730 (Accession # P06763).
Predicted N-terminus: Glu 98
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 74.3 kDa. The protein migrates as 70-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a trimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE

HSV-2 (strain 333) Glycoprotein B (gB), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of HSV-2 (strain 333) Glycoprotein B (gB), His Tag (Cat. No. GLB-H52H3) is more than 95% and the molecular weight of this protein is around 225-255 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized HSV-2 (strain 333) Glycoprotein B (gB), His Tag (Cat. No. GLB-H52H3) at 1 μg/mL (100 μL/well) can bind Mouse anti-Herpes virus 1 and2, clone T111 (Monoclonal), Mouse IgG1 with a linear range of 0.06-0.5 ng/mL (QC tested).
Protocol
Customer Reviews Writing Reviews

Background
Recent Advances
전화
글로벌: +1 800-810-0816 (무료)
아시아태평양: +86 400-682-2521



















