Mouse OX40 Ligand / TNFSF4 Protein, Fc Tag, active trimer, low endotoxin (MALS verified)

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Cat. No. / Size
Price
Qty
OXL-M5263-100ug
$330.00
OXL-M5263-1mg
$2245.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    OX40L, TNFSF4, CD252, Glycoprotein Gp34, TXGP1, CD134 ligand, CD134L

  • Source

    Mouse OX40 Ligand Protein, Fc Tag (OXL-M5263) is expressed from human 293 cells (HEK293). It contains AA Ser 51 - Leu 198 (Accession # P43488-1).

    Predicted N-terminus: Pro

    Request for sequence
  • Molecular Characterization

    OX40 Ligand Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 43.1 kDa. The protein migrates as 43-48 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

    The protein is designed as a trimer.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    OX40 Ligand SDS-PAGE

    Mouse OX40 Ligand Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    OX40 Ligand SEC-MALS

    The purity of Mouse OX40 Ligand Protein, Fc Tag (Cat. No. OXL-M5263) is more than 95% and the molecular weight of this protein is around 258-295 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     OX40 Ligand ELISA

    Immobilized Mouse OX40 Ligand Protein, Fc Tag (Cat. No. OXL-M5263) at 2 μg/mL (100 μL/well) can bind Mouse OX40, His Tag (Cat. No. OX0-M5228) with a linear range of 2-39 ng/mL (QC tested).

    Protocol

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Background

Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L), TAX transcriptionally-activated glycoprotein 1 and CD252, which belongs to the tumor necrosis factor family. TNFSF4 is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. Furthermore, similar to other TNF superfamily members, membrane-bound OX40 Ligand (TNFSF4) exists as a homotrimer. Human TNFSF4 shares 46% amino acid sequence identity with its mouse counterpart.

Recent Advances

 
Drug Development Progress
  • English Name:

    Tumor necrosis factor ligand superfamily member 4

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    13 Details

  • Highest Development Stage:

    Phase 3 Clinical

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