Influenza A virus (Hong Kong/483/97) NP (H5N1) Protein, His Tag

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Cat. No. / Size
Price
Qty
NP1-V52H4-100ug
$350.00
NP1-V52H4-1mg
$2680.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Nucleocapsid protein/NP (Influenza Virus)

  • Source

    Influenza A virus (Hong Kong/483/97) NP (H5N1) Protein, His Tag (NP1-V52H4) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Asn 498 (Accession # AAF74327.1).

    Predicted N-terminus: Met 1

    Request for sequence
  • Molecular Characterization

    Nucleocapsid protein/NP (Influenza Virus) Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 58.1 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Nucleocapsid protein/NP (Influenza Virus) SDS-PAGE

    Influenza A virus (Hong Kong/483/97) NP (H5N1) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     Nucleocapsid protein/NP (Influenza Virus) ELISA

    Immobilized Influenza A virus (Hong Kong/483/97) NP (H5N1) Protein, His Tag (Cat. No. NP1-V52H4) at 1 μg/mL (100 μL/well) can bind Pan Influenza A Nucleoprotein Antibody, Mouse MAb with a linear range of 0.06-1 ng/mL (QC tested).

    Protocol
  • Bioactivity-SPR

     Nucleocapsid protein/NP (Influenza Virus) SPR

    Influenza A virus (Hong Kong/483/97) NP (H5N1) Protein, His Tag (Cat. No. NP1-V52H4) immobilized on CM5 Chip can bind Pan Influenza A Nucleoprotein Antibody, Mouse Mab with an affinity constant of 0.390 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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Background

Influenza, commonly known as ‘the flu’, is an infectious disease of birds and mammals caused by RNA viruses of the family Orthomyxoviridae, the influenza viruses.Influenza viral nucleoprotein (NP) is highly conserved and the most abundant non-enzymatic viral protein in infected cells. NP is a key component of the viral ribonucleoproteins (vRNPs) complex, and its recognized functions include, but are not limited to, binding to RNA and oligomerizing for the vRNP complex, undergoing intracellular trafficking, and participating in the switch from mRNA transcription to vRNA replication.

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