Human Mucin-1 / MUC-1 (890-1158) Protein, His Tag, low endotoxin (MALS verified)

Synonyms

Mucin 1, MUC1, CD227, EMA, H23AG, KL-6, MAM6, MUC-1, SEC, MUC-1, X, MUC1, ZD, PEM, PEMT, PUM, CA15-3, Episialin

Source

Human Mucin-1 (890-1158), His Tag (MU1-H52H9) is expressed from human 293 cells (HEK293). It contains AA Ser 890 - Gly 1158 (Accession # P15941-1).

Predicted N-terminus: Ser 890 (partial α chain) & Ser 1098 (partial β chain)

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus. The mature form of Mucin-1 is a non-covalent heterodimeric complex with the proteolytically cleaved partial α and partial β chain. Each partial α and partial β chain has a calculated MW of 21.3 kDa (partial α chain) and 8.4 kDa (partial β chain). The protein migrates as 40-80 kDa (partial glycosylated α chain) and 10 kDa (partial β chain) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process