PE-Labeled Human MSP1D1 Protein, His Tag (Nanodisc) (Site-specific conjugation)

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제품 번호/규격
가격
수량
APO-HP1E3-25tests
$320.00
APO-HP1E3-200tests
$1792.00
ETA of in-stock products:2 business days
총 항목 수 제품 금액$ 0

제품 세부정보

  • Application

    1. Cell based assay (FACS or functional assay)
    2. Affinity testing (SPR / BLI)
    3. Screening (ELISA)
  • Synonyms

    MSP1D1

  • Source

    Nanodisc PE-Labeled Human MSP1D1 Protein, His Tag (APO-HP1E3) is produced via site-specific conjugation of PE to Human MSP1D1 Protein, His Tag under optimal conditions with a proprietary technology. Human MSP1D1 Protein, His Tag is expressed from E. coli system. It contains AA Ser 1 - Gln 211 (Accession # P11836-1).

    Predicted N-terminus: Met

    Request for sequence

  • Molecular Characterization

    MSP1D1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 29.4 kDa.

    1. Structure

      Nanodisc membrane protein is wrapped by a synthetic phospholipid bilayer membrane structure, composed of membrane scaffold proteins (MSPs) and phospholipids.

    2. Synthesis Process

      Nanodisc reconstitution is a self-assembly process that starts from purified, detergent-solubilized membrane proteins and reconstitutes them into synthetic phospholipid bilayers stabilized by MSP1D1 membrane scaffold protein. This creates a stable, detergent-free environment that preserves native conformation and biological activity, with excellent homogeneity and full compatibility with cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA),etc.

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping and Storage

    This product is supplied and shipped on dry ice.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

고객님도 확인한 상품

데이터 표시

  • Bioactivity-FACS

     MSP1D1 FACS

    5e5 of CD20-CAR-293 cells transfected with anti-CD20-scFv were stained with 100 μL of 1:20 dilution (5 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human MSP1D1 Protein, His Tag (Cat. No. APO-HP1E3) and negative control protein respectively. PE-SA was used as negative control (QC tested).

    Protocol

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배경 소개

A designed thermostable variant of the soluble domain of an ion channel protein, often used in membrane protein structural biology as a fusion partner. Functionally, it relates to mechanosensitive channels. In therapeutic contexts, MSP1D1 serves as a scaffold for generating stable, soluble membrane protein antigens for immunization and antibody discovery.

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