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Product Details
Synonyms
CSF1R, C-FMS, CD115, CSFR, FIM2, FMS, M-CSFR
Source
Human M-CSF R Protein, Mouse IgG2a Fc Tag (CSR-H5255) is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Glu 512 (Accession # P07333-1).
Predicted N-terminus: Ile 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 81.4 kDa. The protein migrates as 105-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human M-CSF R Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human M-CSF R Protein, Mouse IgG2a Fc Tag (Cat. No. CSR-H5255) is more than 85% and the molecular weight of this protein is around 210-230 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human M-CSF R Protein, Mouse IgG2a Fc Tag (Cat. No. CSR-H5255) at 5 μg/mL (100 μL/well) can bind Biotinylated Human M-CSF Protein, His,Avitag (Cat. No. MCF-H82E6) with a linear range of 0.2-3 ng/mL (QC tested).
Protocol
Immobilized Human M-CSF R Protein, Mouse IgG2a Fc Tag (Cat. No. CSR-H5255) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-34 Protein, His,Avitag (Cat. No. IL4-H82E5) with a linear range of 0.2-10 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Colony stimulating factor 1 receptor
- Category：
- Approved Drugs：
11 Details
- Drugs in Clinical Trials：
38 Details
- Highest Development Stage：
Approved
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