Human LRRC32&TGF-beta 1 Heterotrimer protein, His Tag&Tag Free, low endotoxin (MALS verified)

Synonyms

LRRC32 & TGF-beta 1, LRRC32&TGFB1

Source

Human LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Free (GA1-H52W9) is expressed from human 293 cells (HEK293). It contains AA His 20 - Asn 627 (LRRC32) & Leu 30 - Ser 390 (TGF-beta 1) (Accession # Q14392-1 (LRRC32) & P01137-1 (TGF-beta1)).

Predicted N-terminus: Leu 30

Molecular Characterization

Human LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Free (the molar ratio of LRRC32 & TGF-beta 1 equals 1:2) is produced by co-expression of LRRC32 and TGF-beta 1, which has a calculated MW of 68.0 kDa (LRRC32), 28.5 (LAP) and 12.8 kDa (mature TGF-beta 1) respectively. LRRC32 is fused with a polyhistidine tag at the C-terminus and TGF-beta 1 contains no tag. The reducing (R) Heterotrimer protein migrates as 70 kDa (LRRC32), 38-45 kDa (LAP) and 14 kDa (mature TGF-beta 1) when calibrated against Star Ribbon Pre-stained Protein Marker due to glycosylation respectively.

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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