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Product Details
Synonyms
Integrin alpha 2 beta 1, ITGA2 & ITGB1
Source
Human ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52W6) is expressed from human 293 cells (HEK293). It contains AA Tyr 30 - Thr 1132 (ITGA2) & Gln 21 - Asp 728 (ITGB1) (Accession # P17301-1 (E534K) (ITGA2) & P05556-1 (ITGB1)).
Predicted N-terminus: Tyr 30 (ITGA2) & Gln 21 (ITGB1)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Human ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA2 and ITGB1, has a calculated MW of 127.6 kDa (ITGA2) and 83.7 kDa (ITGB1). Subunit ITGA2 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 150-180 kDa (ITGA2) and 100-120 kDa (ITGB1) when calibrated against Star Ribbon Pre-stained Protein Marker respectively due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human Collagen I protein at 2 μg/mL (100 μL/well) can bind Human ITGA2 & ITGB1 Heterodimer Protein (Cat. No. IT1-H52W6) with a linear range of 0.078-2.5μg/mL (QC tested).
Protocol
Immobilized Human ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W6) at 1 μg/mL (100 μL/well) can bind CD49b (Integrin alpha 2) Monoclonal Antibody (AK7), Mouse / IgG1 with a linear range of 0.06-2 ng/mL (QC tested).
Protocol
Immobilized Human COL1A1 Protein, His Tag (Cat. No. CO1-H5243) at 2 μg/mL (100 μL/well) can bind Human ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W6) with a linear range of 0.005-1.25 μg/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Integrin alpha2/beta1
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
1 Details
- Highest Development Stage：
Phase 1 Clinical
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