Golden Hamster IL-23 alpha&IL-12 beta Heterodimer Protein, His Tag&Tag Free, low endotoxin (MALS verified)

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Cat. No. / Size
Price
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ILB-G52W4-50ug
$387.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    IL-23 alpha & IL-12 beta

  • Source

    Golden Hamster IL-23 alpha&IL-12 beta Heterodimer Protein, His Tag&Tag Free (ILB-G52W4) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Ala 196 (IL23A) & Ile 23 - Ser 327 (IL12B) (Accession # A0A1U7QS90 (IL23A) & Q8CJE6 (IL12B)).

    Predicted N-terminus: Leu 20 (IL23A) & Ile 23 (IL12B)

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  • Molecular Characterization

    IL23A & IL12B Structure

    Other Tags and Version Biotin & Other Labeled Version

    The protein has a calculated MW of 21.6 kDa (IL23A) & 34.8 kDa (IL12B). The protein migrates as 21-25 kDa and 38-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

    The protein is designed as a heterodimer.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    IL23A & IL12B SDS-PAGE

    Golden Hamster IL-23 alpha&IL-12 beta Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IL23A & IL12B SEC-MALS

    The purity of Golden Hamster IL-23 alpha&IL-12 beta Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-G52W4) is more than 95% and the molecular weight of this protein is around 55-75 kDa verified by SEC-MALS.

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Background

Interleukin-23 subunit alpha (IL-23A) can associate with interleukin-12 subunit beta (IL-12B) to form IL-23, a heterodimeric cytokine that functions in innate and adaptive immunity. IL-23 promotes the production of IL-17, and together they contribute to acute responses to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the JAK-STAT signaling cascade, stimulates memory T-cells rather than naive T-cells, and promotes the production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may play a key role in autoimmune inflammatory diseases; it may also promote tumorigenesis in certain inflammatory contexts.

Recent Advances

 
Drug Development Progress
  • English Name:

    Interleukin-12 subunit beta

  • Category:

  • Approved Drugs:

    7 Details

  • Drugs in Clinical Trials:

    2 Details

  • Highest Development Stage:

    Approved

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