Human IL-7 R alpha&TSLP R Heterodimer Protein, Fc Tag&Fc Tag, low endotoxin (MALS verified)

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Cat. No. / Size
Price
Qty
ILR-H5255-100ug
$490.00
ILR-H5255-1mg (250ug X 4)
$3385.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    IL7Ra, CD127, TSLP R, CRLF2, IL-XR, ILXR, p2RY8, CRLF2 fusion, TSLP receptor, TSLPR, CRL2, CRLF2Y, Cytokine receptor-like 2

  • Source

    Human IL-7 RA&TSLP R Heterodimer Protein, Fc Tag&Fc Tag (ILR-H5255) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Asp 239 (IL-7 RA) & Gln 23 - Lys 231 (TSLP R) (Accession # P16871-1 (I66T, V138I) (IL-7RA) & Q9HC73-1 (TSLPR)).

    Predicted N-terminus: Glu 21 (IL-7 RA) & Gln 23 (TSLP R)

    Request for sequence
  • Molecular Characterization

    IL-7 R alpha & TSLP R Structure

    Other Tags and Version Biotin & Other Labeled Version

    Human IL-7 RA&TSLP R Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of IL-7 RA and TSLP R, has a calculated MW of 51.7 kDa (IL-7 RA) and 50.3 kDa (TSLP R). Subunit IL-7 RA is fused with a human IgG1 Fc fragment at the C-terminus and subunit TSLP R is fused with a human IgG1 Fc fragment at the C-terminus. The reducing (R) protein migrates as 60-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    IL-7 R alpha & TSLP R SDS-PAGE

    Human IL-7 RA&TSLP R Heterodimer Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IL-7 R alpha & TSLP R SEC-MALS

    The purity of Human IL-7 RA&TSLP R Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILR-H5255) is more than 85% and the molecular weight of this protein is around 115-140 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     IL-7 R alpha & TSLP R ELISA

    Immobilized Human IL-7 RA&TSLP R Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILR-H5255) at 5 μg/mL (100 μL/well) can bind Human TSLP Protein, His Tag, premium grade (Cat. No. TSP-H52Hb) with a linear range of 0.4-3 ng/mL (QC tested).

    Protocol
  •  IL-7 R alpha & TSLP R ELISA

    Serial dilutions of Monoclonal Anti-Human TSLP Antibody, Human IgG2 were added into Human IL-7 RA&TSLP R Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILR-H5255) : Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) binding reactions. The half maximal inhibitory concentration (IC50) is 1.09688 (Routinely tested).

    Protocol
  • Bioactivity-BLI

     IL-7 R alpha & TSLP R BLI

    Loaded Human IL-7 RA&TSLP R Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILR-H5255) on Protein A Biosensor, can bind Human TSLP Protein, His Tag, premium grade (Cat. No. TSP-H52Hb) with an affinity constant of 134 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

    Protocol

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Background

TSLP is a pleiotropic cytokine originally characterized as a lymphocyte growth factor. Thymic stromal lymphopoietin (TSLP) is overexpressed in the airways of severe asthmatics and is an upstream cytokine that orchestrates inflammatory responses in asthma. Human TSLP exerts its biological activities by binding to a high-affinity heteromeric receptor complex composed of TSLPR and IL- 7 receptor and initiates signaling in cells co-expressing TSLPR and IL-7R.

Recent Advances

 
Drug Development Progress
  • English Name:

    Interleukin-7 receptor subunit alpha

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    8 Details

  • Highest Development Stage:

    Phase 2 Clinical

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