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Product Details
Synonyms
IGF-I R / IGF-1 R
Source
Rhesus macaque IGF-I R Protein, His Tag (IGR-R52H3) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # I2CWY3).
Predicted N-terminus: Glu 31 & Asp 741
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin convertase cleavage site, 737-RKRR-740, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.1 kDa and 23.8 kDa respectively. The protein migrates as 110-116 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Rhesus macaque IGF-I R Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Rhesus macaque IGF-I R Protein, His Tag (Cat. No. IGR-R52H3) is more than 85% and the molecular weight of this protein is around 255-311 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Rhesus macaque IGF-I R Protein, His Tag (Cat. No. IGR-R52H3) at 5 μg/mL (100 μL/well) can bind Human IGF-I Protein, Fc Tag (Cat. No. IG1-H5263) with a linear range of 20-156 ng/mL (QC tested).
Protocol
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