HSV-2 (HG52) Glycoprotein E Protein, His Tag (MALS verified)

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Cat. No. / Size
Price
Qty
GLE-H52H4-100ug
$390.00
GLE-H52H4-1mg (500ug X 2)
$2615.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Glycoprotein E/gE (HSV)

  • Source

    HSV-2 (HG52) Glycoprotein E, His Tag (GLE-H52H4) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Arg 414 (Accession # P89475).

    Predicted N-terminus: Ala 21

    Request for sequence
  • Molecular Characterization

    Glycoprotein E/gE (HSV) Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 45.0 kDa. The protein migrates as 55-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Glycoprotein E/gE (HSV) SDS-PAGE

    HSV-2 (HG52) Glycoprotein E, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Glycoprotein E/gE (HSV) SEC-MALS

    The purity of HSV-2 (HG52) Glycoprotein E, His Tag (Cat. No. GLE-H52H4) is more than 90% and the molecular weight of this protein is around 50-65 kDa verified by SEC-MALS.

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  • Bioactivity-ELISA

     Glycoprotein E/gE (HSV) ELISA

    Immobilized HSV-2 (HG52) Glycoprotein E, His Tag (Cat. No. GLE-H52H4) at 1 μg/mL (100 μL/well) can bind anti-HSV-2-gE antibody with a linear range of 0.2-16 ng/mL (QC tested).

    Protocol

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Background

Herpesvirus infections are widely spread throughout the world population. Herpes simplex virus (HSV) belongs to the α-herpesvirus subfamily. There are two main types of HSV, HSV-1 and HSV-2, which infect humans. HSV-2 mainly causes genital lesions, whereas HSV-1 is involved in both oral and genital infections. In epithelial cells, the heterodimer gE/gI is required for the cell-to-cell spread of the virus, by sorting nascent virions to cell junctions. Once the virus reaches the cell junctions, virus particles can spread to adjacent cells extremely rapidly through interactions with cellular receptors that accumulate at these junctions. Implicated in basolateral spread in polarized cells. In neuronal cells, gE/gI is essential for the anterograde spread of the infection throughout the host nervous system.

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