Mouse GDNF / ATF Protein, His Tag, low endotoxin (MALS verified)

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Cat. No. / Size
Price
Qty
GDF-M5245-20ug
$205.00
GDF-M5245-50ug
$310.00
GDF-M5245-500ug
$1855.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    GDNF, ATF

  • Source

    Mouse GDNF, His Tag (GDF-M5245) is expressed from human 293 cells (HEK293). It contains AA Ser 78 - Ile 211 (Accession # P48540).

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  • Molecular Characterization

    GDNF Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 17.0 kDa. The protein migrates as 16 kDa, 18 kDa and 22-24 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Sterility

    Negative

  • Mycoplasma

    Negative

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

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  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    GDNF SDS-PAGE

    Mouse GDNF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    GDNF SEC-MALS

    The purity of Mouse GDNF, His Tag (Cat. No. GDF-M5245) is more than 95% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.

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Background

Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta (TGF-beta) superfamily. It potently promotes the survival of of peripheral and central neurons. It signals through GFRα receptors, particularly GFRα1. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Recent Advances

 
Drug Development Progress
  • English Name:

    Glial cell line-derived neurotrophic factors

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    6 Details

  • Highest Development Stage:

    NDA/BLA

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