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Product Details
Synonyms
Fusion glycoprotein F0/F protein (MuV)
Source
Mumps virus (strain Miyahara vaccine) (MuV) Fusion glycoprotein F0, His Tag (RSF-V52H4) is expressed from human 293 cells (HEK293). It contains AA Val 20 - ILe 486 (Accession # P11236 (FAGIAIGIA 103-111del)).
Predicted N-terminus: Val 20
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 51.9 kDa. The protein migrates as 53-63 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a trimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Mumps virus (strain Miyahara vaccine) (MuV) Fusion glycoprotein F0, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mumps virus (strain Miyahara vaccine) (MuV) Fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H4) is more than 85% and the molecular weight of this protein is around 185-215 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mumps virus (strain Miyahara vaccine) (MuV) Fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Mumps virus Fusion glycoprotein F0 Antibody, Human IgG1 (5A3) (Cat. No. RSF-MY2092) with a linear range of 0.1-4 ng/mL (Routinely tested).
Protocol
Immobilized Mumps virus (strain Miyahara vaccine) (MuV) Fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Mumps virus Fusion glycoprotein F0 Antibody, Human IgG1 (8F8) (Cat. No. RSF-MY2093) with a linear range of 0.06-2 ng/mL (Routinely tested).
Protocol
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