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Product Details
Synonyms
FGF R2 (IIIb), FGFR2B, FGFR2
Source
Human FGF R2 (IIIb), Fc Tag (FGB-H5256) is expressed from human 293 cells (HEK293). It contains AA Pro 154 - Leu 358 (Accession # P21802-3).
Predicted N-terminus: Pro 154
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 49.4 kDa. The protein migrates as 65-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human FGF R2 (IIIb), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) is more than 95% and the molecular weight of this protein is around 119-145 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human FGF basic, premium grade (Cat. No. BFF-H4117) at 5 μg/mL (100 μL/well) can bind Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) with a linear range of 1-16 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) with a linear range of 2-31 ng/mL (Routinely tested).
Protocol
Immobilized Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) at 5 μg/mL (100 μL/well) can bind VENTANA® FGFR2b (FPR2-D) Mouse Monoclonal Antibody with a linear range of 0.06-4 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human FGF-10 Protein, His Tag (Cat. No. FG0-H5147) immobilized on CM5 Chip can bind Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) with an affinity constant of 4.11 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human FGF-5 Protein, His Tag (Cat. No. FG5-H51H3) captured on CM5 chip via anti-His antibody can bind Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) with an affinity constant of 63.3 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) immobilized on CM5 Chip can bind Human FGF-7 Protein, premium grade (Cat. No. FG7-H5213) with an affinity constant of 176 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) on Protein A Biosensor, can bind Human FGF-9, His Tag (Cat. No. FG9-H52H3) with an affinity constant of 119 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Fibroblast growth factor receptor 2
- Category：
- Approved Drugs：
9 Details
- Drugs in Clinical Trials：
32 Details
- Highest Development Stage：
Approved
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