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Product Details
Synonyms
FcRn, FCGRT & B2M
Source
Rat FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (FCM-R5287) is expressed from human 293 cells (HEK293). It contains AA Ala 23 - Ser 298 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P13599-1 (FCGRT) & P07151-1 (B2M)).
Predicted N-terminus: Ala 23 (FCGRT) & Ile 21 (B2M)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Rat FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 31.8 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 43-55 kDa (FCGRT) and 14 kDa (B2M) respectively due to different glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Rat FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Rat FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-R5287) is more than 90% and the molecular weight of this protein is around 55-65 kDa verified by SEC-MALS.
Report
Bioactivity-SPR
Rat FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-R5287) captured on NTA-Series S sensor chip can bind Monoclonal Anti-Her2 Antibody, Human IgG1 (Cat. No.HE2-M598) with an affinity constant of 59.6 nM as determined in a SPR assay (Biacore T200) (QC tested).
Protocol
Rat FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-R5287) captured on NTA-Series S sensor chip can bind Herceptin® with an affinity constant of 1.94 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
IgG receptor FcRn large subunit p51
- Category：
- Approved Drugs：
4 Details
- Drugs in Clinical Trials：
5 Details
- Highest Development Stage：
Approved
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