Human CGRPR&RAMP1 Full Length Protein, His,Flag Tag&Twin-Strep Tag (Detergent)

Application

1. Immunization
2. Affinity testing (SPR / BLI)
3. Screening (ELISA)

Synonyms

CGRPR & RAMP1

Source

Detergent Human CGRPR&RAMP1 Full Length Protein, His,Flag Tag&Twin-Strep Tag (CG1-H52D3) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Asn 461 & Cys 27 - Val 148 (Accession # Q16602-1 & O60894-1).

Predicted N-terminus: Glu 23 & Trp

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

The CGRPR protein carries a polyhistidine tag and a Flag tag at the C-terminus. The RAMP1 protein carries a Twin Strep tag at the N-terminus. The protein has a calculated MW of 69 kDa.

*The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow the link for product information.

1. Structure

   

   Each full-length transmembrane protein is encapsulated by detergent micelle.

2. Synthesis Process

   

   Through mild detergent micelle extraction, dedicated solubilization, and stringent affinity purification, we maximally retain the native transmembrane conformation of full-length transmembrane proteins throughout downstream purification. This workflow yields purified, detergent-solubilized proteins free of unrelated membrane impurities and exogenous scaffold proteins, with exceptional purity, accurate quantitation, and well-preserved biological activity—ideal for immunization, binding assays in antibody discovery and screening (ELISA) and affinity testing (SPR and BLI), etc.

Purity

>90% as determined by SDS-PAGE.

Formulation

This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.

Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, pH7.5, CHS with glycerol as protectant.

Contact us for customized product form or formulation.

Shipping

This product is supplied and shipped with dry ice, please inquire the shipping cost.

Storage

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. The product MUST be stored at -70°C or lower upon receipt;
2. -70°C for 3 months under sterile conditions.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process