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Product Details
Synonyms
CD8 alpha & beta, CD8A & CD8B
Source
Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (CDA-H52W5) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 & Leu 22 - Pro 170 (Accession # P01732 & P10966-1).
Predicted N-terminus: Ser 22 & Leu 22
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
The protein has a calculated MW of 19.5 kDa & 22.1 kDa. The protein migrates as 28-30 kDa and 31-33 kDa under reducing (R) condition, and 30-32 kDa and 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (Cat. No. CDA-H52W5) is more than 85% and the molecular weight of this protein is around 53-68 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (Cat. No. CDA-H52W5) at 2 μg/mL (100 μL/well) can bind Anti-CD8 alpha Antibody, Mouse IgG2a (clone: OKT8) with a linear range of 0.06-4 ng/mL (QC tested).
Protocol
Immobilized Anti-CD8 alpha Antibody, Mouse IgG2a (clone: OKT8) at 1 μg/mL (100 μL/well) can bind Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (Cat. No. CDA-H52W5) with a linear range of 2-39 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Anti-CD8 alpha Antibody, Human IgG1 on Protein A Biosensor, can bind Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (Cat. No. CDA-H52W5) with an affinity constant of 66.8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
T-cell surface glycoprotein CD8 beta chain
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
0 Details
- Highest Development Stage：
Preclinical
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