Product Details
Synonyms
CD47, MER6, IAP, OA3
Source
Canine CD47, His Tag (CD7-C52H5) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Asn 140 (Accession # F1P6D7-1).
Predicted N-terminus: Gln 19
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 15.8 kDa. The protein migrates as 27-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Canine CD47, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA

Immobilized Human SIRP alpha, Fc Tag (HPLC-verified) (Cat. No. SIA-H5251) at 5 μg/mL (100 μL/well) can bind Canine CD47, His Tag (Cat. No. CD7-C52H5) with a linear range of 5-78 ng/mL (QC tested).
Protocol
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Background
Recent Advances
- English Name:
Cluster of differentiation 47
- Category:
- Approved Drugs:
0 Details
- Drugs in Clinical Trials:
53 Details
- Highest Development Stage:
Phase 3 Clinical
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