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Product Details
Synonyms
CD28, Tp44
Source
Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (CD8-H82F2) is expressed from human 293 cells (HEK293). It contains AA Asn 19 - Pro 152 (Accession # AAH93698). In the region Asn 19 - Pro 152, the amino acid sequences of Human, Cynomolgus and Rhesus macaque CD28 are identical.
Predicted N-terminus: Asn 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 43.6 kDa. The protein migrates as 55-70 kDa under reducing (R) condition, and 110-130 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-HPLC
The purity of Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) was greater than 95% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human B7-1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4) with a linear range of 5-78 ng/mL (QC tested).
Protocol
Immobilized Anti-CD28 MAb, Mouse IgG1 at 5 μg/mL (100 μL/well) can bind Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) with a linear range of 2.4-78 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) on SA Biosensor, can bind Human B7-1, Fc Tag (Cat. No. B71-H5259) with an affinity constant of 56.8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) on SA Biosensor, can bind Human B7-1, Mouse IgG2a Fc Tag (Cat. No. B71-H52A4) with an affinity constant of 38.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
T-cell-specific surface glycoprotein CD28
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
31 Details
- Highest Development Stage：
Phase 2 Clinical
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