Biotinylated Human CD20 Full Length Protein, Flag Tag&His,Avitag™ (Nanodisc-pro)

Application

1. Cell based assay (FACS or functional assay)
2. Affinity testing (SPR / BLI)
3. Screening (ELISA)

Synonyms

MS4A1, CD20, MS4A-1

Source

Nanodisc-pro Biotinylated Human CD20 Full Length Protein, Flag Tag&His,Avitag (CD0-H82D3) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Pro 297 (Accession # P11836-1).

Predicted N-terminus: Met

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus followed by an Avi tag (Avitag™). The protein has a calculated MW of 39.1 kDa.

*The isotype control of empty/mock nanodisc (Cat. No. APO-H8113) is sold separately and not included in protein, you can follow the link for product information.

1. Structure

   

   Nanodisc-pro membrane protein is wrapped by a piece of cell membrane and scaffold, the info of scaffold can be found in above red text "isotope type control...".

2. Synthesis Process

   

   Nanodisc-pro membrane protein extraction is a gentle, non-disruptive process that directly isolates full-length transmembrane proteins together with their surrounding native phospholipids from cellular membranes, without breaking or disturbing the original membrane structure. This preserves the membrane protein's natural conformational landscape and native lipid environment, yielding stable nanodisc that retain cellular phospholipids, with fully preserved biological activity—making them ideal for cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA), etc.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the HABA assay / binding ELISA.

Formulation

Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant.

Contact us for customized product form or formulation.

Shipping and Storage

This product is supplied and shipped on dry ice.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. The product MUST be stored at -70°C or lower upon receipt;
2. -70°C for 3 months under sterile conditions.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process