Product Details
Synonyms
CD19, B4, CVID3, MGC12802
Source
Biotinylated Cynomolgus / Rhesus macaque CD19 (20-292) Protein, His,Avitag (CD9-C82E3) is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 292 (Accession # F7F486-1). In the region Pro 20 - Lys 292, the amino acid sequences of Cynomolgus and Rhesus macaque CD19 are identical.
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 33.9 kDa. The protein migrates as 48-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Biotinylated Cynomolgus / Rhesus macaque CD19 (20-292) Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA

Immobilized Biotinylated Cynomolgus / Rhesus macaque CD19 (20-292) Protein, His,Avitag (Cat. No. CD9-C82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Anti-CD19, Human IgG1 with a linear range of 0.02-2.5 μg/mL (QC tested).
Protocol
Immobilized Biotinylated Cynomolgus / Rhesus macaque CD19 (20-292) Protein, His,Avitag (Cat. No. CD9-C82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind various dilution ratio of Rabbit Anti-CD19 Polyclonal Antibody (Routinely tested).
Protocol
Bioactivity-SPR

Biotinylated Cynomolgus / Rhesus macaque CD19 (20‐292) Protein, His,Avitag (Cat. No. CD9‐C82E3) immobilized on SA Chip can bind Monoclonal Anti‐Human CD19 Antibody, Human IgG1 with an affinity constant of 20.0 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
- English Name:
B-lymphocyte antigen CD19
- Category:
- Approved Drugs:
14 Details
- Drugs in Clinical Trials:
435 Details
- Highest Development Stage:
Approved
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