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Product Details
Application
Synonyms
CD133, PROM1, PROML1, Prominin-1, AC133
Source
Nanodisc Human CD133 Full Length Protein, His Tag (CD3-H52H1) is expressed from human 293 cells (HEK293). It contains AA Gly 20 - His 865 (Accession # O43490-1).
Predicted N-terminus: Gly 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
The CD133 carries a polyhistidine tag at the C-terminus with calculated MW of 97.2 kDa and migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
*The isotype control of empty/mock nanodisc (Cat. No. APO-H51H3) is sold separately and not included in protein, you can follow the link for product information.
Structure
Nanodisc membrane protein is wrapped by a synthetic phospholipid bilayer membrane structure, composed of membrane scaffold proteins (MSPs) and phospholipids.
Synthesis Process
Nanodisc reconstitution is a self-assembly process that starts from purified, detergent-solubilized membrane proteins and reconstitutes them into synthetic phospholipid bilayers stabilized by MSP1D1 membrane scaffold protein. This creates a stable, detergent-free environment that preserves native conformation and biological activity, with excellent homogeneity and full compatibility with cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA),etc.
Purity
>90% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
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Performance Data
SDS-PAGE
Human CD133 Full Length Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized Human CD133 Full Length Protein, His Tag (Cat. No. CD3-H52H1) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD133 Antibody, Human IgG1 with a linear range of 0.2-4 ng/mL (QC tested).
Protocol
Bioactivity-FACS
2e5 of Anti-CD133 CAR-293 cells were stained with 100 μL of 3 μg/mL of Human CD133 Full Length Protein, His Tag (Cat. No. CD3-H52H1) and negative control protein respectively, washed and then followed by PE-anti-His Tag antibody and analyzed with FACS (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
CD133
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 2 Clinical
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