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Product Details
Product Details
GENPower™ NLS-Cas9 Nuclease is a recombinant Streptococcus pyogenes Cas9 protein purified from Escherichia coli for CRISPR-based genome editing. The introduction of nuclear localization signals (NLS) can help Cas9 enter the nucleus, increasing the chance of genomic DNA cleavage.
Features and Advantages
- High cleavage activity in different cell line
- High purity (＞95% SDS-PAGE / SEC-MALS)
- Animal-Free materials
- Batch-to-batch consistency
- Stringent quality control tests
Concentration
10 mg/mL
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
Host Cell Protein
≤10 ng/mg of protein tested by ELISA.
Host Cell DNA
≤1 ng/mg of protein tested by qPCR.
Sterility
Negative
Mycoplasma
Negative
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Formulation
Supplied as 0.2 μm filtered solution in 20 mM Tris, 300 mM NaCl, 0.1 mM EDTA, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
This product is stable after storage at:
- The product MUST be stored at -20°C or lower upon receipt;
- -20°C for 18 months under sterile conditions.
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Performance Data
SDS-PAGE
The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of GENPower™ NLS-Cas9 Nuclease, premium grade (Cat. No. CA9-S5149) was greater than 95% as determined by SEC-HPLC.
Bioactivity
GENPower™ NLS-Cas9 Nuclease, premium grade is evaluated in an in vitro DNA cleavage assay on a DNA fragment containing the target sequence. The activity of the GENPower™ NLS-Cas9 Nuclease, premium grade is greater than 90% (QC tested).
The cleavage activity of GENPower™ NLS-Cas9 Nuclease, premium grade in vitro.
The cleavage activity of GENPower™ NLS-Cas9 Nuclease, premium grade in cell line.
The cleavage activity of GENPower™ NLS-Cas9 Nuclease, premium grade in human primary T cells.
The knockout efficiency for B2M in primary T cell was measured by Flow Cytometry.
The knockout efficiency for TRAC in Jurkat cell was measured by Flow Cytometry.
The knockout efficiency for B2M in Jurkat cell was measured by Flow Cytometry.
Bioactivity-Stability
Enzymatic activity assay demonstrates batch-to-batch consistency between Acro's GMP and PG Cas9.
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